ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2018, Vol. 49 ›› Issue (2): 449-458.doi: 10.11843/j.issn.0366-6964.2018.02.025

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Effect of Concentrate-roughage Ratio and Alfalfa Supplementation on Associative Effects of Diets in vitro

YUAN Jiu1*, WAN Xin-jie2, PING Li-ying1, WANG Jun-jun1, HE Tian-le1, YU Hai-shan1   

  1. 1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. Lanzhou Federal Feed Limited Company, Lanzhou 730060, China
  • Received:2017-05-27 Online:2018-02-23 Published:2018-02-23

Abstract:

This experiment was conducted to investigate the effects of wheat straw (WS) or ammonium bicarbonate treated wheat straw (ABWS) basal diets supplemented with different levels of alfalfa on associative effects(AE) in vitro. There were 6 concentrate-roughage ratio(70:30, 60:40, 50:50, 40:60, 30:70, 20:80) and 4 alfalfa supplementation levels (0%, 10%, 20%, 30%) in this study. Roughage contained WS/ABWS and alfalfa. Gas production (GP) of 48 feed combination groups (24 groups of WS and 24 groups of ABWS)and 4 diets was recorded at 2, 4, 6, 9, 12, 24, 36, 48, 72, 96 h for culture. The AE values were calculated by in vitro GP during 24 h and weighted values of different combinations. The results showed as follows:1) In WS basal diet, the AE of 20% group was significantly higher than that of 30%, 10% and 0 groups (P<0.01), the AE of 30% group was significantly higher than that of 10% and 0 groups (P<0.01), the AE of 10% group was significantly higher than that of 0 group (P<0.01) when C:R was 70:30. The AE of 30% group was significantly higher than that of 10% group (P<0.01) and 0 group (P<0.05) when C:R was 50:50. The AE of 30% group was significantly higher than that of 0 group (P<0.01), and the AE of 10% and 20% groups were significantly higher than that of 0 group (P<0.05) when C:R was 40:60. 2) In ABWS basal diet, the AE of 30% and 10% groups were significantly higher than that of 0 group (P<0.05) when C:R was 70:30, the AE of 10% group was significantly higher than that of 30% group (P<0.05) when C:R was 60:40, the AE of 10%, 20% and 0 groups were significantly higher than that of 30% group (P<0.01) when C:R was 50:50, the AE of 0 group was significantly higher than that of 20% group (P<0.05) when C:R was 40:60. It is concluded that ABWS diet need less alfalfa (10%) than WS (30%, 20%) diet to develop positive AE. ABWS is better than WS in reduing the supplement of alfalfa.

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